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Automated Bioreactor Feeding with the Groton ARS-M, DASGIP Parallel Bioreactor System and the YSI 7100 is featured in the BioProcess International Industry Yearbook.
August 18, 2009
Bioprocess monitoring and automatic feeding of key nutrients such as glucose and amino acids are critical to obtain optimal, high productivity in cell culture systems. Here we present a complete, closed loop system that integrates a Groton Biosystems Automated Reactor Sample System with a nutrient analyzer and a reactor control system that demonstrates a powerful, automated online, real time feeding system.
Materials and Methods:
A Groton Biosystems Automated Reactor Sampler (ARS-M) is connected to a 4 vessel DasGip Parallel Bioreactor System (PBS, Jülich, Germany) controlled by the DasGip Control 4.0 OPC Edition Software (DCS) and an YSI7100 Biochemistry Analyzer (YSI, Yellow Springs, Ohio). Data and control were transmitted between the individual units of the Integrated System by OPC protocol.
Glucose was chosen as the feedstock. The ARS-M delivered on a programmed schedule an aliquot of reactor media to the nutrient monitor. The glucose concentration data point was transmitted to the ARS-M which transformed the data point in OPC format. The reactor control system OPC client polled automatically and repetitively the ARS OPC server to acquire new data as it became available. The reactor control system then operated on the data to calculate a new feed stock flow rate to satisfy the selected set point value. Figure 1 shows a schematic of the system set-up.
In this demonstration, the system was programmed to acquire samples from four reactors with a single reactor sample and analysis cycle performed in 10 minutes. The setpoint was selected as 2 gm/L glucose. The reactor control system feedback algorithm was configured to achieve and maintain a steady state glucose set point (2 g/L) as shown in the figure.
Click here to download copy - http://www.grotonbiosystems.com/library/docs/MKT-165_BioProcess_Article_July_2009.pdf
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